Abstract

Marine ecosystems are recognized as rich reservoirs of bioactive pigment-producing microorganisms with potential industrial applications. In this study, an orange-pigmented bacterium was isolated from marine sediment collected from Colachel, Tamil Nadu, India, and identified as Planococcus maritimus KA01 through 16S rRNA sequencing and phylogenetic analysis. Optimization of culture conditions showed that maximum carotenoid production occurred at 25 °C, pH 7, after 96 h of incubation, with 1.0% NaCl, glucose as the carbon source, and under shaking conditions. Methanol showed the highest efficiency solvent for pigment extraction. Spectral characterization via UV-Vis, FTIR, and GC-MS suggested the presence of carotenoid-like compounds, with squalene identified as a major metabolite. The pigment extract exhibited antibacterial activity, with lower MIC and MBC values against Staphylococcus aureus compared with Pseudomonas aeruginosa, indicating greater susceptibility of Gram-positive bacteria under the tested conditions. Antioxidant analysis using the DPPH assay demonstrated dose-dependent radical-scavenging activity. Overall, the study suggests that P. maritimus KA01 is a promising source of carotenoid-containing pigments with antimicrobial and antioxidant properties. Further studies involving purification, toxicity assessment, and large-scale production are required to establish potential applications.

Keywords

Planococcus Maritimus, Marine Bacterium, Carotenoid Pigment, Antibacterial Activity, Antioxidant Activity,

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References

  1. J.V.D.O. Barreto, L. M. Casanova, A.N. Junior, M.C.P.P. Reis-Mansur, A.B. Vermelho, Microbial pigments: Major Groups and Industrial Applications. Microorganisms, 11(12), (2023) 2920. https://doi.org/10.3390/microorganisms11122920
  2. H.B. Slama, A. Chenari Bouket, Z. Pourhassan, F.N. Alenezi, A. Silini, H. Cherif-Silini, T. Oszako, L. Luptakova, P. Golińska, L. Belbahri, Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences, 11(14), (2021) 6255. https://doi.org/10.3390/app11146255
  3. B.L. Tusset, I. Mocelin, L.C. Villa, A.E.T. dos Santos, R. de Matos, L. Kmetzsch, F.C. Lopes, The Microbial Palette: From Bioprospecting to Genetic Engineering of Microbial Pigments. Fermentation, 12(6), (2026) 263. https://doi.org/10.3390/fermentation12060263
  4. R. Panesar, S. Kaur, P.S. Panesar, Production of Microbial Pigments Utilizing Agro-Industrial Waste: a Review. Current Opinion in Food Science, 1, (2015) 70-76. https://doi.org/10.1016/j.cofs.2014.12.002
  5. P. Rajendran, P. Somasundaram, L. Dufossé, Microbial Pigments: Eco-Friendly Extraction Techniques and Some Industrial Applications. Journal of Molecular Structure, 1290, (2023) 135958. https://doi.org/10.1016/j.molstruc.2023.135958
  6. L. Dufossé, Microbial Pigments from Bacteria, Yeasts, Fungi, and Microalgae for the Food and Feed Industries. In Natural and Artificial Flavoring Agents and Food Dyes, (2018) 113-132. https://doi.org/10.1016/B978-0-12-811518-3.00004-1
  7. M. Numan, S. Bashir, R. Mumtaz, S. Tayyab, N.U. Rehman, A.L. Khan, Z.K. Shinwari, A. Al-Harrasi, Therapeutic Applications of Bacterial Pigments: a Review of Current Status and Future Opportunities. 3 Biotech, 8(4), (2018) 207. https://doi.org/10.1007/s13205-018-1227-x
  8. C.K. Venil, L. Dufossé, P. Renuka Devi, Bacterial Pigments: Sustainable Compounds with Market Potential for Pharma and Food Industry. Frontiers in Sustainable Food Systems, 4, (2020) 100. https://doi.org/10.3389/fsufs.2020.00100
  9. M. Rodrigo-Baños, I. Garbayo, C. Vílchez, M.J. Bonete, R.M. Martínez-Espinosa Carotenoids from Haloarchaea and Their Potential in Biotechnology. Marine Drugs, 13(9), (2015) 5508-5532. https://doi.org/10.3390/md13095508
  10. J. Torregrosa-Crespo, Z. Montero, J.L. Fuentes, M. Reig Garcia-Galbis, I. Garbayo, C. Vílchez, R.M. Martínez-Espinosa, Exploring the Valuable Carotenoids for the Large-Scale Production by marine Microorganisms. Marine Drugs, 16(6), (2018) 203. https://doi.org/10.3390/md16060203
  11. C. Galasso, C. Corinaldesi, C. Sansone Carotenoids from Marine Organisms: Biological Functions and Industrial Applications. Antioxidants, 6(4), (2017) 96. https://doi.org/10.3390/antiox6040096
  12. X. Che, P. Ma, M. Chen, W. Fan, A comprehensive review of development and utilization of Marine Microorganisms as New Food Resources. Journal of Future Foods, (2025) https://doi.org/10.1016/j.jfutfo.2025.08.020
  13. S. Neagu, M.M. Stancu, Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production. Biotech, 14(2), (2025) 49. https://doi.org/10.3390/biotech14020049
  14. S. Waghmode, M. Suryavanshi, D. Sharma, S.K. Satpute, Planococcus Species–An Imminent Resource to Explore Biosurfactant and Bioactive Metabolites for Industrial Applications. Frontiers in Bioengineering and Biotechnology, 8, (2020) 996. https://doi.org/10.3389/fbioe.2020.00996
  15. N. Pallath, B. Francis, S. Devanesan, K. Farhat, M. Balakrishnan, Isolation and characterization of novel carotenoid pigment from marine Planococcus maritimus MBP-2 and their biological applications. Journal of King Saud University-Science, 35(8), (2023) 102872. https://doi.org/10.1016/j.jksus.2023.102872
  16. S. Gemelli, S.T. Silveira, M.E. Pailliè-Jiménez, A.D.O. Rios, A. Brandelli, Production, Extraction and Partial Characterization of Natural Pigments from Chryseobacterium sp. kr6 Growing on Feather Meal Biomass. Biomass, 4(2), (2024) 530-542. https://doi.org/10.3390/biomass4020028
  17. N. Siddharthan, R. Sandhiya, N. Hemalatha, Extraction and Characterization of Antibacterial Pigment from Roseomonas Gilardii YP1 Strain in Yercaud Soil. Asian Journal of Pharmaceutical and Clinical Research, 13(3), (2020) 116–120. https://doi.org/10.22159/ajpcr.2020.v13i3.36580
  18. S.B. Chaturwedi, S. Mainali, R. Chaudhary, Antibacterial Activity of Pigment Extracted from Bacteria Isolated from Soil Samples. BMC Research Notes, 17(1), (2024) 169. https://doi.org/10.1186/s13104-024-06834-4
  19. S.B. Chaturwedi, S. Mainali, R. Chaudhary, Antibacterial Activity of Pigment Extracted from Pigment Producing Bacteria. Research Square, (2023). https://doi.org/10.21203/rs.3.rs-2522714/v1
  20. I. Wiegand, K. Hilpert, R.E.W. Hancock, Agar and Broth Dilution Methods to Determine the Minimal Inhibitory Concentration (MIC) of Antimicrobial Substances. Nature Protocols, 3(2), (2008) 163-175. https://doi.org/10.1038/nprot.2007.521
  21. B. Zhang, R. Yang, G. Zhang, Y. Liu, D. Zhang, W. Zhang, T. Chen, G. Liu, Characteristics of Planococcus Antioxidans sp. nov., an Antioxidant‐Producing Strain Isolated from the Desert Soil in the Qinghai–Tibetan Plateau. MicrobiologyOpen, 9(6), (2020) 1183-1196. https://doi.org/10.1002/mbo3.1028
  22. D. Zhao, N. Zeng, D. Wang, B. Li, G. Yu, C. Li, Exploring the Influence Mechanism of Low/High Temperatures on Carotenoid Production in Sporobolomyces Pararoseus: Insights from Physiological and Transcriptomic Analyses. Biotechnology and Bioengineering, 122(7), (2025) 1615-1626 https://doi.org/10.1002/bit.28985
  23. L. Singh, D. Prusty, M. Behera, K. Perveen, N.A. Bukhari, Optimizing pH and Light for Enhanced Carotenoid Synthesis and Antioxidant Properties in Sub‐Aerial Cyanobacteria. Journal of Basic Microbiology, 65(2), (2025) e2400570. https://doi.org/10.1002/jobm.202400570
  24. S. Allahkarami, A.A. Sepahi, H. Hosseini, M.R. Razavi, Isolation and Identification of Carotenoid-Producing Rhodotorula sp. from Pinaceae Forest Ecosystems and Optimization of in Vitro Carotenoid Production. Biotechnology Reports, 32, (2021) e00687. https://doi.org/10.1016/j.btre.2021.e00687
  25. A.S. Fernandes, S.M. Paixão, T.P. Silva, J.C. Roseiro, L. Alves, Influence of Culture Conditions Towards Optimal Carotenoid Production by Gordonia Alkanivorans Strain 1B. Bioprocess and Biosystems Engineering, 41(2), (2018) 143-155. https://doi.org/10.1007/s00449-017-1853-4
  26. V. Kumar, S. Agrawal, S.K. Shahi, A. Motghare, S. Singh, P.C. Ramamurthy, Bioremediation Potential of Newly Isolated Bacillus Albus Strain VKDS9 for Decolourization and Detoxification of Biomethanated Distillery Effluent and its Metabolites Characterization for Environmental Sustainability. Environmental Technology and Innovation, 26, (2022) 102260. https://doi.org/10.1016/j.eti.2021.102260
  27. S. Ram, M. Mitra, F. Shah, S.R. Tirkey, S. Mishra, Bacteria as an Alternate Biofactory for Carotenoid Production: A review of its Applications, Opportunities and Challenges. Journal of Functional Foods, 67, (2020) 103867. https://doi.org/10.1016/j.jff.2020.103867
  28. S.D. Murkute, V. Joshi, P. Shete, A. Jain, N.P. Patil, Disparity in Pigmented Halophilic Bacterial Communities Across Diverse Reserves of Saline Ecosystem. The Microbe, 8, (2025) 100459. https://doi.org/10.1016/j.microb.2025.100459
  29. L.D. Silva-Arias, L. Díaz, E. Coy-Barrera, Bioproduction Optimization, Characterization, and Bioactivity of Extracellular Pigment Produced by Streptomyces Parvulus. International Journal of Molecular Sciences, 26(21), (2025) 10762. https://doi.org/10.3390/ijms262110762
  30. M. Styczynski, A. Rogowska, K. Gieczewska, M. Garstka, A. Szakiel, L. Dziewit, Genome-Based Insights into the Production of Carotenoids by Antarctic Bacteria, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B. Molecules, 25(19), (2020) E4357. https://doi.org/10.3390/molecules25194357
  31. Y.S. Liu, J.Y. Wu, K.P. Ho, Characterization of Oxygen Transfer Conditions and their effects on Phaffia Rhodozyma Growth and Carotenoid Production in Shake-Flask Cultures. Biochemical Engineering Journal, 27(3), (2006) 331–335. https://doi.org/10.1016/j.bej.2005.08.031
  32. E.L. Ashenafi, M.C. Nyman, J.T. Shelley, N.S. Mattson, Spectral Properties and Stability of Selected Carotenoid and Chlorophyll Compounds in Different Solvent Systems. Food Chemistry Advances, 2, (2023) 100178. https://doi.org/10.1016/j.focha.2022.100178
  33. G. Britton, Structure and Properties of Carotenoids in Relation to Function. FASEB Journal, 9(15), (1995) 1551–1558. https://doi.org/10.1096/fasebj.9.15.8529834
  34. T.A. Telegina, Y.L. Vechtomova, A.V. Aybush, A.A. Buglak, M.S. Kritsky, Isomerization of Carotenoids in Photosynthesis and Metabolic Adaptation. Biophysical Reviews, 15(5), (2023) 887–906. https://doi.org/10.1007/s12551-023-01156-4
  35. M. Rohde, The Gram-Positive Bacterial Cell Wall. Microbiology Spectrum, 7(3), (2019) 3–18. https://doi.org/10.1128/microbiolspec.GPP3-0044-2018
  36. J. Flieger, M. Raszewska-Famielec, E. Radzikowska-Büchner, W. Flieger, Skin Protection by Carotenoid Pigments. International Journal of Molecular Sciences, 25(3), (2024) 1431. https://doi.org/10.3390/ijms25031431
  37. C. Santana-Molina, V. Henriques, D. Hornero-Méndez, D.P. Devos, E. Rivas-Marin, The Squalene Route to C30 Carotenoid Biosynthesis and the Origins of Carotenoid Biosynthetic Pathways. Proceedings of the National Academy of Sciences, 119(52), (2022) e2210081119. https://doi.org/10.1073/pnas.2210081119
  38. S. Waghmode, M. Suryavanshi, L. Dama, S. Kansara, V. Ghattargi, P. Das, A. Banpurkar, S.K. Satpute, Genomic Insights of Halophilic Planococcus Maritimus SAMP MCC 3013 and Detail Investigation of its Biosurfactant Production. Frontiers in Microbiology, 10, (2019) 235. https://doi.org/10.3389/fmicb.2019.00235
  39. J.M.E. Aranda, C.S. Lazana, FT-IR Spectral Characterization of Aromatic Compounds in Pyrolytic Oil from Waste Tires: Implications for Alternative Fuel Applications. Journal of Electrical and Electronics Research, 2(1), (2025) 1–8. https://doi.org/10.64820/aepjeer.21.1.8.62025
  40. E. Fuente, J.A. Menéndez, M.A. Díez, D. Suárez, M.A. Montes-Morán, Infrared Spectroscopy of Carbon Materials: A Quantum Chemical Study of Model Compounds. The Journal of Physical Chemistry B, 107(26), (2003) 6350–6359. https://doi.org/10.1021/jp027482g
  41. Y. Chen, B. Xie, J. Yang, J. Chen, Z. Sun, Identification of Microbial Carotenoids and Isoprenoid Quinones from Rhodococcus sp. B7740 and its Stability in the Presence of Iron in Model Gastric Conditions. Food Chemistry, 240, (2018) 204–211. https://doi.org/10.1016/j.foodchem.2017.06.067
  42. A. Ventosa, J.J. Nieto, A. Oren, Biology of Moderately Halophilic Aerobic Bacteria. Microbiology and Molecular Biology Reviews: MMBR, 62(2), (1998) 504–544. https://doi.org/10.1128/mmbr.62.2.504-544.1998
  43. B. Tang, K.H. Row, Development of Gas Chromatography Analysis of Fatty Acids in Marine Organisms. Journal of Chromatographic Science, 51(7), (2013) 599–607. https://doi.org/10.1093/chromsci/bmt005
  44. J. Wang, R. Zhang, X. Chen, X. Sun, Y. Yan, X. Shen, Q. Yuan, Biosynthesis of Aromatic Polyketides in Microorganisms using Type II Polyketide Synthases. Microbial Cell Factories, 19(1), (2020) 110. https://doi.org/10.1186/s12934-020-01367-4
  45. Z. Breijyeh, B. Jubeh, R. Karaman, Resistance of Gram-Negative Bacteria to Current Antibacterial Agents and Approaches to Resolve it. Molecules, 25(6), (2020) E1340. https://doi.org/10.3390/molecules25061340
  46. S. Neagu, M.M. Stancu, Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production. BioTech, 14(2) (2025) 49. https://doi.org/10.3390/biotech14020049
  47. R.F. Langendonk, D.R. Neill, J.L. Fothergill, The Building Blocks of Antimicrobial Resistance in Pseudomonas Aeruginosa: Implications for Current Resistance-Breaking Therapies. Frontiers in Cellular Infection Microbiology, 11, (2021) 665759. https://doi.org/10.3389/fcimb.2021.665759
  48. N.T. Kulasekaran, M.L. JT, V. Vasavan, D. Gopal, J. Marimuthu, Improved Total Carotenoid Content from Planococcus Plakortidis NIOT3 through Microwave Assisted Extraction and genome-guided Pathway Annotation. Enzyme and Microbial Technology, 190(110707), (2025) 110707. https://doi.org/10.1016/j.enzmictec.2025.110707
  49. S.M. Huszczynski, J.S. Lam, C.M. Khursigara, The Role of Pseudomonas Aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology. Pathogens, 9(1), (2019) E6. https://doi.org/10.3390/pathogens9010006
  50. H.F. Ji, Insight into the Strong Antioxidant Activity of Deinoxanthin, a Unique Carotenoid in Deinococcus Radiodurans. International Journal of Molecular Sciences, 11(11), (2010) 4506–4510. https://doi.org/10.3390/ijms11114506
  51. M.S. Blois, Antioxidant Determinations by the use of a Stable Free Radical. Nature, 181(4617), (1958) 1199-1200. https://doi.org/10.1038/1811199a0